In Silico and In Vitro Study of The Ethanol Extract of The White Garland Lily (Hedychium coronarium J. Koenig) as a Tyrosinase Inhibitor

The Zingiberaceae family includes the White Garland Lily (Hedychium coronarium J. Koenig). It is used in traditional medicine for fever, analgesics, and indigestion. In current study, silico prediction of active compound from Hedychium coronarium, analysis interaction with a tyrosinase inhibitor, testing inhibitor using dopachrome compounds were done. software docking simulation Autodock-GPU. Kojic acid solution containing 1000, 500, 250, 125, 62.5 ppm was measured into 96-well microtiter dish to which enzyme added, pH adjusted phosphate buffer. mixture incubated at 37ºC 30 minutes before absorbance wavelength 492 nm microplate reader. Triparanol molecules have best bonding affinity molecular data, -8.00 kcal/mol value. extract yield 3.88%, total ash content 12.60% ± 0.27%, acid-insoluble 0.23% 0.02%, water 5.96%. has potential be developed as Tyrosinase result insilico investigation. Based on result, ethanol inhibited an IC50 value 183.85 compared kojic 17.09 ppm.